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Human PD-L1/B7-H1 enzyme-linked immunoassay kit (EH0048)

Price: Regular price $499.00

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Catalog# EH0048

Assay type Sandwich ELISA

Standard curve range 40.96~10000 pg/ml

Sensitivity 8.50 pg/ml (50 μl)；26.02 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02400.02720.0256
40.960.03570.04310.03940.0138
102.400.05840.06370.06110.0355
256.000.10990.10930.10960.0840
640.000.23890.23770.23830.2127
1600.000.61450.64340.62900.6034
4000.001.63501.72901.68201.6564
10000.003.62903.83003.72953.7039

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)209.21061.12965.9236.71178.33104.7
Standard Deviation13.160.9181.015.650.0169.9
Coefficient of Variation(%)6.35.76.16.64.25.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-124.466336.72

Sensitivity

8.50 pg/ml (50 μl)；26.02 pg/ml (10 μl)

Standard curve range

40.96~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human PD-L1 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 96% to 127% with an overall mean recovery of 117%.

Specifications

Product Type

ELISA Kit

Product Name

Human PD-L1/B7-H1 enzyme-linked immunoassay kit (EH0048)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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