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Human Osteoprotegerin/TNFRSF11B enzyme-linked immunoassay kit (EH0070)

Price: Regular price $499.00

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Catalog# EH0070

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 0.37 pg/ml (50 μl)；2.53 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01490.01390.0144
6.860.03320.03090.03210.0171
20.580.06860.06810.06840.0534
61.730.17820.17700.17760.1626
185.190.49470.49590.49530.4803
555.561.27001.26501.26751.2525
1666.672.68002.54202.61102.5960
5000.003.84303.69703.77003.7550

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)194.3749.01558.3209.4735.11459.6
Standard Deviation12.235.0105.39.624.829.6
Coefficient of Variation(%)6.34.76.84.63.42.0

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30341.28- 586.42100461.05

Sensitivity

0.37 pg/ml (50 μl)；2.53 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Osteoprotegerin into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 65% to 98% with an overall mean recovery of 95%.

Specifications

Product Type

ELISA Kit

Product Name

Human Osteoprotegerin/TNFRSF11B enzyme-linked immunoassay kit (EH0070)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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