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Human Osteopontin(OPN) enzyme-linked immunoassay kit (EH0057)

Price: Regular price $499.00

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Catalog# EH0057

Assay type Sandwich ELISA

Standard curve range 0.31~20 ng/ml

Sensitivity 0.04 ng/ml (50 μl)；0.12 ng/ml (10 μl)

Reacts with

  • Human

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

ng/ml O.D. Average Corrected
0.000.01650.01760.0171
0.310.03630.03960.03800.0209
0.630.07490.08600.08050.0634
1.250.17780.19550.18670.1696
2.500.42250.46200.44230.4252
5.001.01101.05401.03251.0155
10.002.03502.13202.08352.0665
20.003.50403.41203.45803.4410

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(ng/ml)0.422.096.220.512.106.03
Standard Deviation0.020.090.480.020.100.37
Coefficient of Variation(%)4.94.57.74.34.76.1

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum301.1-6.741003.97

Sensitivity

0.04 ng/ml (50 μl)；0.12 ng/ml (10 μl)

Standard curve range

0.31~20 ng/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human OPN into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 83% to 117% with an overall mean recovery of 108%.

Specifications

Product Type

ELISA Kit

Product Name

Human Osteopontin(OPN) enzyme-linked immunoassay kit (EH0057)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

1 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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