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Human MMP-9 enzyme-linked immunoassay kit (EH0020)

Price: Regular price $499.00

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Catalog# EH0020

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 2.7 pg/ml (50 μl)；7.9 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01430.0140.0142
13.720.02120.0210.02110.0070
41.150.03310.03340.03330.0191
123.460.07120.06730.06930.0551
370.370.18120.17280.17700.1629
1111.110.46920.47760.47340.4593
3333.331.23251.19871.21561.2015
10000.002.71412.63552.67482.6607

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)225.21135.83363.1234.01080.93328.7
Standard Deviation6.458.6182.612.316.5111.9
Coefficient of Variation(%)2.85.25.45.31.53.4

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30203.3-765.1100428.1

Sensitivity

2.7 pg/ml (50 μl)；7.9 pg/ml (10 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human MMP-9 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 89% to 114% with an overall mean recovery of 101%.

Specifications

Product Type

ELISA Kit

Product Name

Human MMP-9 enzyme-linked immunoassay kit (EH0020)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 piecse

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