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Human MICA enzyme-linked immunoassay kit (EH0044)

Price: Regular price $499.00

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Catalog# EH0044

Assay type Sandwich ELISA

Standard curve range 156.25~10000 pg/ml

Sensitivity 8.30 pg/ml (50 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02020.02140.0208
156.250.08080.08280.08180.0610
312.500.14300.14400.14350.1227
625.000.26820.26610.26720.2464
1250.000.51250.52940.52100.5002
2500.001.01601.03701.02651.0057
5000.001.96701.94101.95401.9332
10000.003.29503.41803.35653.3357

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)389.91231.63586.9373.185.43682.9
Standard Deviation21.245.6111.94.641.585.4
Coefficient of Variation(%)5.43.73.12.53.12.3

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30469.25-3717.251001351.05

Sensitivity

8.30 pg/ml (50 μl)

Standard curve range

156.25~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human MICA into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 94% to 112% with an overall mean recovery of 106%.

Specifications

Product Type

ELISA Kit

Product Name

Human MICA enzyme-linked immunoassay kit (EH0044)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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