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Human Mesothelin enzyme-linked immunoassay kit(one step) (EHY0152)

Price: Regular price $629.00

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Catalog# EHY0152

Assay type Sandwich ELISA

Standard curve range 1.37~1000 ng/ml

Sensitivity 0.16 ng/ml (50 μl)；0.23 ng/ml (10 μl)；

Reacts with

  • Human

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

ng/ml O.D. Average Corrected
0.000.01280.01500.0139
1.370.02310.02350.02330.0094
4.120.04550.04420.04490.0310
12.350.11270.11870.11570.1018
37.040.31420.30750.31090.2970
111.110.88700.94680.91690.9030
333.332.55502.44202.49852.4846
1000.004.32964.42734.37854.3646

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(ng/ml)22.2107.5332.819.397.4295.0
Standard Deviation0.84.513.40.83.59.2
Coefficient of Variation(%)3.54.24.03.93.53.1

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum3051.87-224.14100119.25

Sensitivity

0.16 ng/ml (50 μl)；0.23 ng/ml (10 μl)；

Standard curve range

1.37~1000 ng/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Mesothelin into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 85% to 103% with an overall mean recovery of 94%.

Specifications

Product Type

ELISA Kit

Product Name

Human Mesothelin enzyme-linked immunoassay kit(one step) (EHY0152)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

Q13421

PROTEIN NAME

Mesothelin

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