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Human Leptin Receptor enzyme-linked immunoassay kit (EH0187)

Price: Regular price $499.00

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Catalog# EH0187

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 0.90 pg/ml (50 μl)；5.07 pg/ml (10 μl)；

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00880.00920.0090
13.720.02080.02100.02090.0119
41.150.05390.05140.05270.0437
123.460.14800.14440.14620.1372
370.370.46460.41200.43830.4293
1111.111.16801.18401.17601.1670
3333.332.52502.46402.49452.4855
10000.004.10754.11264.11014.1011

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)272.7915.83089.71303.1985.93109.3
Standard Deviation13.942.7174.5111.446.1189.2
Coefficient of Variation(%)5.14.75.63.84.76.1

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum3024.77-61.5810037.42

Sensitivity

0.90 pg/ml (50 μl)；5.07 pg/ml (10 μl)；

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Leptin Receptor into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 106% with an overall mean recovery of 93%.

Specifications

Product Type

ELISA Kit

Product Name

Human Leptin Receptor enzyme-linked immunoassay kit (EH0187)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

TARGET

UNIPROT #

P48357

PROTEIN NAME

Leptin

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