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Human Leptin enzyme-linked immunoassay kit (EH0056)

Price: Regular price $499.00

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Catalog# EH0056

Assay type Sandwich ELISA

Standard curve range 40.96~10000 pg/ml

Sensitivity 9.45 pg/ml (50 μl)；18.83 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00900.00900.0090
40.960.01290.01360.01330.0043
102.400.02110.02080.02100.0120
256.000.05490.05440.05470.0457
640.000.23270.22070.22670.2177
1600.001.00600.95580.98090.9719
4000.002.70702.67402.69052.6815
10000.003.88503.82403.85453.8455

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)220.91012.43975.4245.91135.24163.7
Standard Deviation4.830.5203.413.167.5262.1
Coefficient of Variation(%)2.23.05.15.35.96.3

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30393.86-7522.161004332.2

Sensitivity

9.45 pg/ml (50 μl)；18.83 pg/ml (10 μl)

Standard curve range

40.96~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Leptin into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 89% to 118% with an overall mean recovery of 104%.

Specifications

Product Type

ELISA Kit

Product Name

Human Leptin enzyme-linked immunoassay kit (EH0056)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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