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Human IL-8/CXCL8 enzyme-linked immunoassay kit (EH0014)

Price: Regular price $499.00

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Catalog# EH0014

Assay type Sandwich ELISA

Standard curve range 4.12~3000 pg/ml

Sensitivity 1.45 pg/ml (50 μl)；3.63 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.03810.03730.0377
4.120.04930.05330.05130.0136
12.350.07430.07850.07640.0387
37.040.15830.16040.15940.1217
111.110.37730.37670.37700.3393
333.330.92940.94470.93710.8994
1000.002.32562.34242.33402.2963
3000.004.01714.13824.07774.0400

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)54.3279.3896.151.2259.7780.4
Standard Deviation1.98.331.33.414.225.7
Coefficient of Variation(%)3.43.03.56.75.53.3

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3043.4-359.3100127.2

Sensitivity

1.45 pg/ml (50 μl)；3.63 pg/ml (10 μl)

Standard curve range

4.12~3000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-8 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 84% to 117% with an overall mean recovery of 102%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-8/CXCL8 enzyme-linked immunoassay kit (EH0014)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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