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Human IL-7 enzyme-linked immunoassay kit (EH0045)

Price: Regular price $499.00

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Catalog# EH0045

Assay type Sandwich ELISA

Standard curve range 2.74~2000 pg/ml

Sensitivity 0.62 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01390.01440.0142
2.740.02210.02020.02120.007
8.230.03470.03470.03470.021
24.690.08170.07300.07740.063
74.070.21220.19760.20490.191
222.220.64470.59790.62130.607
666.671.68101.58801.63451.620
2000.003.70703.59103.64903.635

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)40.2198.2606.441.1221.2654.7
Standard Deviation2.111.219.12.410.621.7
Coefficient of Variation(%)5.35.63.15.94.83.3

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-30.802510.94

Sensitivity

0.62 pg/ml (10 μl)

Standard curve range

2.74~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-7 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 96% with an overall mean recovery of 88%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-7 enzyme-linked immunoassay kit (EH0045)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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