Human IL-6 enzyme-linked immunoassay kit (EH0001)
Catalog# EH0001
Assay type Sandwich ELISA
Standard curve range 1.37~1000 pg/ml
Sensitivity 0.26 pg/ml (50 μl) ；0.43 pg/ml (10 μl)
Reacts with
-
Human
This product has been cited in peer reviewed publications, see list HERE
PERFORMANCE CHARACTERISTICS
Product detail
| pg/ml | O.D. | Average | Corrected | |
|---|---|---|---|---|
| 0 | 0.021 | 0.024 | 0.0225 | |
| 1.37 | 0.0293 | 0.0281 | 0.0287 | 0.0062 |
| 4.12 | 0.0454 | 0.0467 | 0.0461 | 0.0236 |
| 12.35 | 0.096 | 0.0964 | 0.0962 | 0.0737 |
| 37.04 | 0.271 | 0.2485 | 0.2598 | 0.2373 |
| 111.11 | 0.682 | 0.6878 | 0.6849 | 0.6624 |
| 333.33 | 1.8285 | 1.7097 | 1.7691 | 1.7466 |
| 1000 | 3.7268 | 3.8787 | 3.8028 | 3.7803 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 24 | 115.4 | 263.2 | 24.5 | 132.1 | 353.3 |
| Standard Deviation | 1.3 | 4.5 | 9.3 | 2 | 5.9 | 18.4 |
| Coefficient of Variation(%) | 5.6 | 3.9 | 3.6 | 6.2 | 4.4 | 5.2 |
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 2.12-6.46 | 100 | 3.53 |
Sensitivity
0.26 pg/ml (50 μl) ；0.43 pg/ml (10 μl)
Standard curve range
1.37~1000 pg/ml
Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-6 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 84% to 115% with an overall mean recovery of 99%.
Specifications
Product Type
ELISA Kit
Product Name
Human IL-6 enzyme-linked immunoassay kit (EH0001)
Detection method
Colorimetric
Sample type
Serum
Assay type
Sandwich ELISA
Category
Fully Ready-to-Use
PROPERTIES
Storage instructions
Store at +4℃
Kits Components
Coated Plate
96 well
Standard
9 vial
Detect Antibody
6 ml/bottle
Streptavidin-HRP
12 ml/bottle
Assay Buffer
12 ml/bottle
Stop Solution
12 ml/bottle
Wash Buffer
50 ml/bottle
Sealer Film
6 pieces
