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Human IL-5 enzyme-linked immunoassay kit (EH0008)

Price: Regular price $499.00

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Catalog# EH0008

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.15 pg/ml (50 μl) ；0.73 pg/ml (10 μl)

Reacts with

  • Human

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
00.03240.03030.0314
1.370.03920.03460.03690.0056
4.120.04840.04660.04750.0162
12.350.08320.08180.08250.0512
37.040.17650.16620.17140.14
111.110.44540.42450.4350.4036
333.331.10391.07971.09181.0605
10002.86452.85482.85972.8283

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)16.377.9275.816.479.9291.5
Standard Deviation0.42.411.70.31.78.9
Coefficient of Variation(%)2.73.14.22.12.13.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-6.72212.13

Sensitivity

0.15 pg/ml (50 μl) ；0.73 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-5 into health human serum sample. The un-spiked serum was used as blank in these experiments. The recovery ranged from 88% to 115% with an overall mean recovery of 106%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-5 enzyme-linked immunoassay kit (EH0008)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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