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Human IL-33 enzyme-linked immunoassay kit (EH0047)

Price: Regular price $499.00

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Catalog# EH0047

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.76 pg/ml (50 μl)；1.25 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02540.02680.0261
1.370.03260.03170.03220.0061
4.120.04750.04020.04390.0178
12.350.07360.07320.07340.0473
37.040.17100.17300.17200.1459
111.110.48270.48510.48390.4578
333.331.38001.39501.38751.3614
1000.003.62603.62203.62403.5979

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)22.7109.9312.723.1111.8322.4
Standard Deviation1.03.414.91.11.87.6
Coefficient of Variation(%)4.53.14.84.81.62.4

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-16.51316.51

Sensitivity

0.76 pg/ml (50 μl)；1.25 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-33 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 99% to 127% with an overall mean recovery of 108%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-33 enzyme-linked immunoassay kit (EH0047)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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