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Human IL-3 enzyme-linked immunoassay kit (EH0050)

Price: Regular price $499.00

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Catalog# EH0050

Assay type Sandwich ELISA

Standard curve range 40.96~10000 pg/ml

Sensitivity 3.74 pg/ml (50 μl)；20.64 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01570.01590.0158
40.960.03470.03600.03540.0240
102.400.06250.06500.06380.0488
256.000.13950.13910.13930.1243
640.000.32460.31030.31750.3025
1600.000.80320.80160.80240.7874
4000.001.91001.90301.90651.8915
10000.003.27703.19203.23453.2195

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)244.61239.73546.7191.8849.42573.0
Standard Deviation21.5110.5309.97.331.2141.2
Coefficient of Variation(%)8.88.98.73.83.75.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-40.963720.6

Sensitivity

3.74 pg/ml (50 μl)；20.64 pg/ml (10 μl)

Standard curve range

40.96~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-3 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 165% to 197% with an overall mean recovery of 184%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-3 enzyme-linked immunoassay kit (EH0050)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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