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Human IL-21 enzyme-linked immunoassay kit (EH0018)

Price: Regular price $499.00

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Catalog# EH0018

Assay type Sandwich ELISA

Standard curve range 78.13~5000 pg/ml

Sensitivity 21.7 pg/ml (50 μl)；67.9 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01420.01410.0142
78.130.01890.02110.02000.0059
156.250.03470.03310.03390.0198
312.500.06710.07210.06960.0555
625.000.18740.18210.18480.1706
1250.000.57110.55130.56120.5471
2500.001.51131.48611.49871.4846
5000.003.30913.22213.26563.2515

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)86.4416.31244.6102.4444.91444.7
Standard Deviation7.024.168.09.015.2107.9
Coefficient of Variation(%)8.15.85.58.83.47.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-132.64278.8

Sensitivity

21.7 pg/ml (50 μl)；67.9 pg/ml (10 μl)

Standard curve range

78.13~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-21 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 93% to 124% with an overall mean recovery of 109%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-21 enzyme-linked immunoassay kit (EH0018)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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