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Human IL-18 enzyme-linked immunoassay kit (EH0053)

Price: Regular price $499.00

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Catalog# EH0053

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 1.38 pg/ml (50 μl)；2.13 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.03960.03810.0389
6.860.04930.04710.04820.0094
20.580.06990.06810.06900.0302
61.730.12680.12970.12830.0894
185.190.31770.30740.31260.2737
555.560.87500.87710.87610.8372
1666.672.43702.43502.43602.3972
5000.004.54984.62084.58534.5465

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)1439.9447.684.61253.3395.775.1
Standard Deviation34.813.94.1105.222.95.2
Coefficient of Variation(%)2.43.14.88.76.66.1

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum302.04-14.771009.97

Sensitivity

1.38 pg/ml (50 μl)；2.13 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-18 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 97% to 130% with an overall mean recovery of 109%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-18 enzyme-linked immunoassay kit (EH0053)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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