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Human IL-17E/IL-25 enzyme-linked immunoassay kit (EH0066)

Price: Regular price $499.00

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Catalog# EH0066

Assay type Sandwich ELISA

Standard curve range 2.74~2000 pg/ml

Sensitivity 0.69 pg/ml (50 μl)；2.74 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01120.01170.0115
2.740.01800.01790.01800.0065
8.230.03210.03080.03150.0200
24.690.07100.06890.07000.0585
74.070.20000.18080.19040.1790
222.220.57390.55330.56360.5522
666.671.62501.49201.55851.5471
2000.003.67203.55703.61453.6031

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)40.3180.8566.438.9186.5589.6
Standard Deviation2.010.257.12.38.835.4
Coefficient of Variation(%)4.95.710.15.84.76.0

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-4.62202.30

Sensitivity

0.69 pg/ml (50 μl)；2.74 pg/ml (10 μl)

Standard curve range

2.74~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-17E/IL-25 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 117% with an overall mean recovery of 98%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-17E/IL-25 enzyme-linked immunoassay kit (EH0066)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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