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Human IL-17A enzyme-linked immunoassay kit (EH0004)

Price: Regular price $499.00

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Catalog# EH0004

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.26 pg/ml (50 μl) ；0.65 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
00.02190.02090.0214
1.370.02610.02610.02610.0047
4.120.03790.03950.03870.0173
12.350.07710.0850.08110.0597
37.040.17990.17330.17660.1552
111.110.51780.52590.52190.5005
333.331.39291.41091.40191.3805
10003.28883.27943.28413.2627

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)27.6113.6265.523.9125.5324.1
Standard Deviation0.95.311.60.84.28.2
Coefficient of Variation(%)3.24.64.43.33.32.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-10.72402.03

Sensitivity

0.26 pg/ml (50 μl) ；0.65 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-17A into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 112% with an overall mean recovery of 96%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-17A enzyme-linked immunoassay kit (EH0004)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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