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Human IL-15 enzyme-linked immunoassay kit (EH0031)

Price: Regular price $499.00

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Catalog# EH0031

Assay type Sandwich ELISA

Standard curve range 0.69~500 pg/ml

Sensitivity 0.14 pg/ml (50 μl)；0.79 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00900.00900.0090
0.690.02010.01900.01960.0106
2.060.04600.04190.04400.0350
6.170.11110.10450.10780.0988
18.520.32200.29370.30790.2989
55.560.85090.80280.82690.8179
166.672.05011.81771.93391.9249
500.003.52303.41393.46853.4595

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)10.038.9102.916.170.4166.2
Standard Deviation0.51.85.00.94.78.5
Coefficient of Variation(%)4.84.74.95.86.75.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum301.17-4.911002.5

Sensitivity

0.14 pg/ml (50 μl)；0.79 pg/ml (10 μl)

Standard curve range

0.69~500 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-15 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 142% to 189% with an overall mean recovery of 173%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-15 enzyme-linked immunoassay kit (EH0031)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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