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Human IL-13 enzyme-linked immunoassay kit (EH0049)

Price: Regular price $499.00

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Catalog# EH0049

Assay type Sandwich ELISA

Standard curve range 312.5~20000 pg/ml

Sensitivity 38.56 pg/ml (50 μl)；179.32 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01700.01540.0162
312.500.04080.03970.04030.0241
625.000.07630.07600.07620.0600
1250.000.17930.18470.18200.1658
2500.000.45470.45100.45290.4367
5000.001.18611.18051.18331.1671
10000.002.59822.63272.61552.5993
20000.004.06284.16054.11174.0955

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)454.62142.16330.9450.72260.86366.3
Standard Deviation21.444.0120.217.9120.8174.4
Coefficient of Variation(%)4.72.11.94.05.32.7

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-16.7216.712.91

Sensitivity

38.56 pg/ml (50 μl)；179.32 pg/ml (10 μl)

Standard curve range

312.5~20000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-13 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 107% to 114% with an overall mean recovery of 111%.

Specifications

Product Type

ELISA Kit

Product Name

Human IL-13 enzyme-linked immunoassay kit (EH0049)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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