Human IL-1 beta Recombinant Rabbit Monoclonal Antibody [PS01-39] (HA721712)
Catalog# HA721712
Rabbit Monoclonal to Human IL-1 beta
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WB
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Human
Western blot analysis of Human IL-1 beta on different lysates with Rabbit anti-Human IL-1 beta antibody (HA721712) at 1/1,000 dilution.
Lane 1: THP-1 whole cell lysate
Lane 2: THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 31 kDa
Observed band size: 31/28 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721712) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Human IL-1 beta Recombinant Rabbit Monoclonal Antibody [PS01-39] (HA721712)
Immunogen
Recombinant protein within full length human il-1 beta.
Host
Rabbit
Positive Control
THP-1 treated with 80nM TPA overnight then treated with 100ng/mL LPS for 6 hours and 300ng/mL BFA for 3 hours whole cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PS01-39
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 31 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Human IL-1 beta
SYNONYMS
Catabolin antibody;H1 antibody;IFN beta inducing factor antibody;IL 1 antibody;IL 1 beta antibody;IL-1 beta antibody;IL1 antibody;IL1 BETA antibody;IL1B antibody;IL1B_HUMAN antibody;IL1F2 antibody;Interleukin 1 beta antibody;Interleukin 1 beta precursor antibody;interleukin 1, beta antibody;Interleukin-1 beta antibody;OAF antibody;Osteoclast activating factor antibody;OTTHUMP00000162031 antibody;Preinterleukin 1 beta antibody;Preinterleukin beta antibody;Pro interleukin 1 beta antibody
SUBCELLULAR LOCATION
Cytoplasm, Lysosome, Secreted
FUNCTION
Interleukin-1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β. Increased production of IL-1β causes a number of different autoinflammatory syndromes, most notably the monogenic conditions referred to as Cryopyrin-Associated Periodic Syndromes (CAPS), due to mutations in the inflammasome receptor NLRP3 which triggers processing of IL-1B. Intestinal dysbiosis has been observed to induce osteomyelitis through a IL-1β dependent manner. The presence of IL-1β has been also found in patients with multiple sclerosis (a chronic autoimmune disease of the central nervous system). However, it is not known exactly which cells produce IL-1β. Treatment of multiple sclerosis with glatiramer acetate or natalizumab has also been shown to reduce the presence of IL-1β or its receptor.[18]
CITATIONS
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Inhibition of P2X7 Purinergic Receptor Ameliorates Cardiac Fibrosis by Suppressing NLRP3/IL-1β Pathway
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WB
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Mouse
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Citation
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