Human IgM Recombinant Rabbit Monoclonal Antibody [JF84-09] (ET1702-81)
Catalog# ET1702-81
Rabbit Monoclonal to Human IgM
-
WB
-
IHC-P
-
IP
-
FC
-
IF-Cell
-
Human
Lysates/proteins at 20 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 80 kDa
Exposure time: 30 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1702-81) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Human IgM Recombinant Rabbit Monoclonal Antibody [JF84-09] (ET1702-81)
Immunogen
Recombinant protein within human igm from human serum (sigma#i8260).
Host
Rabbit
Positive Control
Daudi cell lysate, human IgM protein, human tonsil tissue, human spleen tissue, Daudi.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JF84-09
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:5,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:1,000
-
IF-Cell
-
1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 50 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Human IgM
SYNONYMS
Immunoglobin heavy chain constant region mu edit item name - Immunoglobin heavy chain mu constant region antibody;Immunoglobin heavy chain constant region mu antibody;AGM1 antibody;Constant region of heavy chain of IgM antibody;DKFZp686I15196 antibody;DKFZp686I15212 antibody;FLJ00385 antibody;Ig mu chain C region antibody;IGHM antibody;IgM heavy chain constant region antibody;Immunoglobin heavy constant mu antibody;Immunoglobulin mu antibody;MGC104996 antibody;MGC52291 antibody;MU antibody;VH antibody
SUBCELLULAR LOCATION
Secreted, Cell membrane.
FUNCTION
Immunoglobulin M (IgM) is one of several isotypes of antibody (also known as immunoglobulin) that are produced by vertebrates. IgM is the largest antibody, and it is the first antibody to appear in the response to initial exposure to an antigen. In humans and other mammals that have been studied, Plasmablasts residing in the spleen are the main source for specific IgM production. IgM interacts with several other physiological molecules: IgM can bind complement component C1 and activate the classical pathway, leading to opsonization of antigens and cytolysis; IgM binds to the polyimmunoglobulin receptor (pIgR) in a process that brings IgM to mucosal surfaces, such as the gut lumen and into breast milk. This binding depends on J chain; Two other Fc receptors that bind IgM—Fcα/µ-R and Fcµ-R -- have been detected. Fcα/µ-R, like pIgR, binds polymeric IgM and IgA. Fcα/µ-R can mediate endocytosis, and its expression in the gut suggests a role in mucosal immunity. Fcµ-R (formerly known as Toso/Faim3) binds IgM exclusively and can mediate cellular uptake of IgM-conjugated antigen.
CITATIONS
-
Screening differentially expressed genes between endometriosis and ovarian cancer to find new biomarkers for endometriosis
-
IF
-
Human
-
Citation
-
