Human IgG heavy chain Recombinant Mouse Monoclonal Antibody [54-76-R] (HA601283)
Catalog# HA601283
Mouse Monoclonal to Human IgG heavy chain
Tested for
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WB
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ELISA
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IHC-P
Reacts with
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Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Human IgG heavy chain on Recombinant Human IgG protein with Mouse anti-Human IgG heavy chain antibody (HA601283) at 1/1,000 dilution.
Lysates/proteins at 50 ng/Lane.
Observed band size: 50 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 50 ng/Lane.
Observed band size: 50 kDa
Exposure time: 15 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.