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Human IDO1 enzyme-linked immunoassay kit (EH0118)

Price: Regular price $499.00

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Catalog# EH0118

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 1.61 pg/ml (50 μl)；3.04 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01800.01620.0171
6.860.02630.02680.02660.0095
20.580.04550.04280.04420.0271
61.730.09970.09410.09690.0798
185.190.26840.25170.26010.2430
555.560.78690.71860.75280.7357
1666.671.93901.87201.90551.8884
5000.003.66603.70503.68553.6684

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)119.6549.61673.2108.1542.61597.0
Standard Deviation5.633.690.32.922.263.4
Coefficient of Variation(%)4.76.15.42.74.14.0

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3062.75-652.09100291.93

Sensitivity

1.61 pg/ml (50 μl)；3.04 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IDO1 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 103% with an overall mean recovery of 92%.

Specifications

Product Type

ELISA Kit

Product Name

Human IDO1 enzyme-linked immunoassay kit (EH0118)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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