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Human ICAM-1 / CD54 enzyme-linked immunoassay kit (EH0064)

Price: Regular price $499.00

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Catalog# EH0064

Assay type Sandwich ELISA

Standard curve range 41.15~30000 pg/ml

Sensitivity 0.49 pg/ml (50 μl)；6.36 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00730.00730.0073
41.150.00920.00950.00940.0021
123.460.01670.01490.01580.0085
370.370.03890.03680.03790.0306
1111.110.14370.14130.14250.1352
3333.330.69500.67740.68620.6789
10000.002.13502.07002.10252.0952
30000.003.73203.60303.66753.6602

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)750.82955.88132.1699.43236.18913.5
Standard Deviation38.6163.5460.829.1188.7535.4
Coefficient of Variation(%)5.15.55.74.25.86.0

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum30111.33-174.86100140.01

Sensitivity

0.49 pg/ml (50 μl)；6.36 pg/ml (10 μl)

Standard curve range

41.15~30000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human ICAM-1 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 97% to 103% with an overall mean recovery of 99%.

Specifications

Product Type

ELISA Kit

Product Name

Human ICAM-1 / CD54 enzyme-linked immunoassay kit (EH0064)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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