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Human Granzyme B enzyme-linked immunoassay kit (EH0052)

Price: Regular price $499.00

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Catalog# EH0052

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 1.12 pg/ml (50 μl)；5.96 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01470.01400.0144
6.860.02750.02630.02690.0126
20.580.04530.04530.04530.0310
61.730.10440.10230.10340.0890
185.190.25620.25650.25640.2420
555.560.70010.70000.70010.6857
1666.671.67701.68001.67851.6642
5000.003.06603.06703.06653.0522

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)97.1448.01618.584.9453.41514.2
Standard Deviation5.117.458.53.710.031.0
Coefficient of Variation(%)5.23.93.64.32.22.1

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum309.62-835.77100230.34

Sensitivity

1.12 pg/ml (50 μl)；5.96 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Granzyme B into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 102% to 112% with an overall mean recovery of 110%.

Specifications

Product Type

ELISA Kit

Product Name

Human Granzyme B enzyme-linked immunoassay kit (EH0052)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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