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Human Granzyme A/GZMA enzyme-linked immunoassay kit (EH0129)

Price: Regular price $499.00

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Catalog# EH0129

Assay type Sandwich ELISA

Standard curve range 2.74~2000 pg/ml

Sensitivity 0.46 pg/ml (50 μl)；1.46 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01360.01410.0139
2.740.02550.02390.02470.0109
8.230.03920.04100.04010.0263
24.690.08450.08240.08350.0696
74.070.21410.21120.21270.1988
222.220.62530.59110.60820.5944
666.671.79101.74401.76751.7537
2000.003.78303.79003.78653.7727

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)49.6198.7609.847.5189.0608.9
Standard Deviation2.38.726.31.57.320.0
Coefficient of Variation(%)4.64.44.33.23.93.3

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3062.47-179.55100.0113.58

Sensitivity

0.46 pg/ml (50 μl)；1.46 pg/ml (10 μl)

Standard curve range

2.74~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human Granzyme A into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 97% with an overall mean recovery of 91%.

Specifications

Product Type

ELISA Kit

Product Name

Human Granzyme A/GZMA enzyme-linked immunoassay kit (EH0129)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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