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Human GM-CSF enzyme-linked immunoassay kit (EH0051)

Price: Regular price $499.00

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Catalog# EH0051

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 3.57 pg/ml (50 μl)；5.45 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00820.00820.0082
13.720.02600.02660.02630.0181
41.150.06370.06990.06680.0586
123.460.18060.17680.17870.1705
370.370.46660.49230.47950.4713
1111.111.20901.27601.24251.2343
3333.332.30602.57702.44152.4333
10000.003.38603.56903.47753.4693

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)195895.92513.3183.5915.42844.3
Standard Deviation11.943.4183.310.945.6159.5
Coefficient of Variation(%)6.14.87.35.95.05.6

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-3.96213.02

Sensitivity

3.57 pg/ml (50 μl)；5.45 pg/ml (10 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human GM-CSF into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 75% to 100% with an overall mean recovery of 84%.

Specifications

Product Type

ELISA Kit

Product Name

Human GM-CSF enzyme-linked immunoassay kit (EH0051)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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