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Human FGF2/b-FGF, C-His, Flag Tag Protein (Active) (HA210582)

Price: Regular price $379.00

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Catalog# HA210582

Biologically active: yes

Reacts with

  • Human

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Product Details

Product Type

Protein

Product Name

Human FGF2/b-FGF, C-His, Flag Tag Protein (Active) (HA210582)

Predicted molecular mass

19.7 kD

Protein construction description

A DNA sequence encoding the human FGF2/b-FGF protein (P09038) (Pro 143-Ser 288) was expressed with both His, Flag tag at the C-terminus.

Accession

P09038

Protein construction

FGF2/b-FGF (143-288) | His | Flag

Source

E.coli

Bio Activity

Measured in a cell proliferation assay using NIH/3T3 mouse fibroblast cells. The ED50 for this effect is ≤0.01 ng/mL.

Purity

>90% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4, 5% Trehalose, 5% mannitol.

Species

Human

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.

Usage Guide

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped with blue ice. Bulk packages of recombinant proteins are provided as frozen liquid which are shipped with dry ice.

Storage

Please avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -20C to -80C It is recommended that aliquot the reconstituted solution to minimize freeze-thaw cycles.

Reconstitution

Reconstitute at 250 μg/ml in sterile water.

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