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Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit (EH0024)

Price: Regular price $499.00

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Catalog# EH0024

Assay type Sandwich ELISA

Standard curve range 2.74~2000 pg/ml

Sensitivity 1.51 pg/ml (50 μl)；9.93 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01500.01500.0150
2.740.02100.02200.02150.0065
8.230.02950.03100.03030.0153
24.690.06010.05500.05760.0426
74.070.16200.14570.15390.1389
222.220.57400.49560.53480.5198
666.672.20351.98792.09572.0807
2000.004.56854.49104.52984.5148

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)36.8179.7464.231.66170.64581.98
Standard Deviation1.46.913.82.3811.5925.24
Coefficient of Variation(%)3.93.837.56.84.3

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum305.45-24.7410015.01

Sensitivity

1.51 pg/ml (50 μl)；9.93 pg/ml (10 μl)

Standard curve range

2.74~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human FGF into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 78% to 92% with an overall mean recovery of 86%.

Specifications

Product Type

ELISA Kit

Product Name

Human FGF basic /FGF2/bFGF enzyme-linked immunoassay kit (EH0024)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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