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Human EGF enzyme-linked immunoassay kit (EH0023)

Price: Regular price $499.00

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Catalog# EH0023

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.12 pg/ml (50 μl)；0.59 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01150.01210.0118
1.370.02930.02970.02950.0177
4.120.06760.06830.06800.0562
12.350.16330.16750.16540.1536
37.040.45360.45520.45440.4426
111.111.14531.19461.17001.1582
333.332.30652.44912.37782.3660
1000.003.24123.28253.26193.2501

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)13.261.3217.820.4105.0347.9
Standard Deviation0.54.16.61.05.319.3
Coefficient of Variation(%)3.56.73.04.95.15.6

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30200.7-417.0100304.2

Sensitivity

0.12 pg/ml (50 μl)；0.59 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human EGF into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 78% to 106% with an overall mean recovery of 93%.

Specifications

Product Type

ELISA Kit

Product Name

Human EGF enzyme-linked immunoassay kit (EH0023)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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