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Human CXCL1/GRO alpha enzyme-linked immunoassay kit (EH0054)

Price: Regular price $499.00

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Catalog# EH0054

Assay type Sandwich ELISA

Standard curve range 20.48~5000 pg/ml

Sensitivity 14.89 pg/ml (50 μl)；32.67 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01370.01290.0133
20.480.01700.01610.01660.0033
51.200.02610.02340.02480.0115
128.000.04460.04430.04450.0312
320.000.14920.14740.14830.1350
800.000.48570.50210.49390.4806
2000.001.25701.26301.26001.2467
5000.002.27602.25102.26352.2502

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)119.3509.01409.9110.0510.11498.1
Standard Deviation9.439.3109.45.427.0100.4
Coefficient of Variation(%)7.97.77.84.95.36.7

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3077.28-616.13100358.62

Sensitivity

14.89 pg/ml (50 μl)；32.67 pg/ml (10 μl)

Standard curve range

20.48~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human CXCL1 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 72% to 125% with an overall mean recovery of 101%.

Specifications

Product Type

ELISA Kit

Product Name

Human CXCL1/GRO alpha enzyme-linked immunoassay kit (EH0054)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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