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Human CD80 enzyme-linked immunoassay kit(EH0121)

Price: Regular price $499.00

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Catalog# EH0121

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.08 pg/ml (50 μl)；0.52 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01070.01070.0107
1.370.02940.02830.02890.0182
4.120.06490.06480.06490.0542
12.350.18010.17610.17810.1674
37.040.51490.51140.51320.5025
111.111.37101.42401.39751.3868
333.333.09503.24903.17203.1613
1000.004.26954.38104.32534.3146

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)20.9106.6336.522.2109.1333.8
Standard Deviation1.27.232.80.73.411.9
Coefficient of Variation(%)3.96.85.93.03.23.6

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3017.39-112.3210070.39

Sensitivity

0.08 pg/ml (50 μl)；0.52 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human B7-1/CD80 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 81% to 111% with an overall mean recovery of 99%.

Specifications

Product Type

ELISA Kit

Product Name

Human CD80 enzyme-linked immunoassay kit(EH0121)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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