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Human CD27/TNFRSF7 enzyme-linked immunoassay kit (EH0114)

Price: Regular price $499.00

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Catalog# EH0114

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 18.37 pg/ml (50 μl)；3.98 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02110.02190.0215
13.720.03460.03090.03280.0113
41.150.07080.06620.06850.0470
123.460.14910.13870.14390.1224
370.370.39970.35020.37500.3535
1111.111.07001.10501.08751.0660
3333.332.78002.75702.76852.7470
10000.004.47934.41184.44564.4241

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)235.31382.04675.0207.71101.33437.3
Standard Deviation16.290.3227.310.368.9259.5
Coefficient of Variation(%)6.96.54.95.06.37.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30114.75-12084.571006777.71

Sensitivity

18.37 pg/ml (50 μl)；3.98 pg/ml (10 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human CD27/TNFRSF7 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 118% with an overall mean recovery of 103%.

Specifications

Product Type

ELISA Kit

Product Name

Human CD27/TNFRSF7 enzyme-linked immunoassay kit (EH0114)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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