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Human CCL5/RANTES enzyme-linked immunoassay kit (EH0027)

Price: Regular price $499.00

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Catalog# EH0027

Assay type Sandwich ELISA

Standard curve range 2.74~2000 pg/ml

Sensitivity 0.7 pg/ml (50 μl)；4.65 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01660.01750.0171
2.740.02500.02730.02620.0091
8.230.04110.03830.03970.0227
24.690.08900.09270.09090.0738
74.070.29200.28750.28980.2727
222.221.06601.12301.09451.0775
666.673.19103.38703.28903.2720
2000.004.28074.39404.33744.3203

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(ng/ml)0.31.56.60.31.56.7
Standard Deviation0.00.10.30.00.10.4
Coefficient of Variation(%)5.03.44.95.15.65.4

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum305.88-31.8710019.53

Sensitivity

0.7 pg/ml (50 μl)；4.65 pg/ml (10 μl)

Standard curve range

2.74~2000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human CCL5 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 73% to 124% with an overall mean recovery of 97%.

Specifications

Product Type

ELISA Kit

Product Name

Human CCL5/RANTES enzyme-linked immunoassay kit (EH0027)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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