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Human CCL25 enzyme-linked immunoassay kit (EH0117)

Price: Regular price $499.00

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Catalog# EH0117

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.06 pg/ml (50 μl)；0.32 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00670.00630.0065
1.370.02120.02060.02090.0144
4.120.05350.05120.05240.0459
12.350.15450.15100.15280.1463
37.040.44500.42920.43710.4306
111.111.21101.23801.22451.2180
333.332.65202.63602.64402.6375
1000.004.01704.03524.02614.0196

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)23.6103.0292.724.1115.1349.9
Standard Deviation1.05.418.01.31.819.4
Coefficient of Variation(%)4.45.36.25.31.65.5

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum300.12-5.851003.15

Sensitivity

0.06 pg/ml (50 μl)；0.32 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human CCL25 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 77% to 98% with an overall mean recovery of 84%.

Specifications

Product Type

ELISA Kit

Product Name

Human CCL25 enzyme-linked immunoassay kit (EH0117)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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