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Human beta-NGF enzyme-linked immunoassay kit (EH0065)

Price: Regular price $499.00

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Catalog# EH0065

Assay type Sandwich ELISA

Standard curve range 6.86~5000 pg/ml

Sensitivity 1.91 pg/ml (50 μl)；3.27 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01720.01640.0168
6.860.02280.02340.02310.0063
20.580.04180.04050.04120.0244
61.730.10040.10160.10100.0842
185.190.26760.29570.28170.2649
555.560.75390.81590.78490.7681
1666.671.94501.96401.95451.9377
5000.003.34003.76103.55053.5337

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)188.01692.32668.5204.0977.62851.8
Standard Deviation11.474.7142.211.843.6106.6
Coefficient of Variation(%)6.04.45.35.84.53.7

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-17.9287.57.23

Sensitivity

1.91 pg/ml (50 μl)；3.27 pg/ml (10 μl)

Standard curve range

6.86~5000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human beta-NGF into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 104% to 132% with an overall mean recovery of 117%.

Specifications

Product Type

ELISA Kit

Product Name

Human beta-NGF enzyme-linked immunoassay kit (EH0065)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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