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Human B7-H2/CD275 enzyme-linked immunoassay kit (EH0133)

Price: Regular price $499.00

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Catalog# EH0133

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 0.01 pg/ml (50 μl)；4.95 pg/ml (10 μl)

Reacts with

  • Human

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.01440.01260.0135
13.720.03360.03430.03400.0205
41.150.08070.07250.07660.0631
123.460.18940.22740.20840.1949
370.370.49510.55510.52510.5116
1111.111.28801.36001.32401.3105
3333.332.72602.83602.78102.7675
10000.004.17224.22584.19904.1855

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)246.61218.84221.3132.7829.43607.5
Standard Deviation19.397.6239.45.463.9268.3
Coefficient of Variation(%)7.88.05.74.07.77.4

Sample Values

Sample MatrixSample EvaluatedRange (ng/ml)Detectable (%) Mean of Detectable (ng/ml)
Serum300.07-193.97100106.56

Sensitivity

0.01 pg/ml (50 μl)；4.95 pg/ml (10 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human B7-H2/CD275 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 72% to 107% with an overall mean recovery of 87%.

Specifications

Product Type

ELISA Kit

Product Name

Human B7-H2/CD275 enzyme-linked immunoassay kit (EH0133)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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