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Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit (EH0203)

Price: Regular price $499.00

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Catalog# EH0203

Assay type Sandwich ELISA

Standard curve range 156.25~10000 pg/ml

Sensitivity 6.88 pg/ml (50 μl)；32.49 pg/ml (10 μl)；

Reacts with

  • Human

  • Monkey

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.00710.00680.0070
156.250.02400.02290.02350.0165
312.500.04540.04760.04650.0396
625.000.10400.10620.10510.0982
1250.000.27390.28480.27940.2724
2500.000.75210.78440.76830.7613
5000.001.92401.91101.91751.9106
10000.003.65103.66803.65953.6526

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(None)184.3899.02654.9187.9893.62794.1
Standard Deviation14.156.692.16.737.536.0
Coefficient of Variation(%)7.66.33.53.64.21.3

Sample Values

Sample MatrixSample EvaluatedRange (None)Detectable (%) Mean of Detectable (None)
Serum30969.07-5411.951002341.85

Sensitivity

6.88 pg/ml (50 μl)；32.49 pg/ml (10 μl)；

Standard curve range

156.25~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human alpha Fetoprotein into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 86% to 110% with an overall mean recovery of 95%.

Specifications

Product Type

ELISA Kit

Product Name

Human/Monkey alpha Fetoprotein enzyme-linked immunoassay kit (EH0203)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

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