PRODUCT CODE: ET1609-73

HtrA2/Omi Recombinant Rabbit Monoclonal Antibody [ST54-05] (ET1609-73)

  • Recombinant

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of HtrA2 on 293 cell lysates using anti-HtrA2 antibody at 1/1,000 dilution.
  • Western blot analysis of HtrA2 on 293 cell lysates using anti-HtrA2 antibody at 1/1,000 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HtrA2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HtrA2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-HtrA2 antibody. Counter stained with hematoxylin.
Western blot analysis of HtrA2 on 293 cell lysates using anti-HtrA2 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

HtrA2/Omi Recombinant Rabbit Monoclonal Antibody [ST54-05] (ET1609-73)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293, human liver tissue, human colon cancer tissue, mouse liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

ST54-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

38 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

HtrA2/Omi

SYNONYMS

High temperature requirement protein A2 antibody; HTRA 2 antibody; HtrA like serine protease antibody; HtrA serine peptidase 2 antibody; HtrA, E. coli, homolog of, 2 antibody; HtrA2 antibody; HTRA2_HUMAN antibody; mitochondrial antibody; Omi stress regulated endoprotease antibody; Omi stress-regulated endoprotease antibody; PARK 13 antibody; PARK13 antibody; Protease serine 25 antibody; PRSS 25 antibody; PRSS25 antibody; Serine protease 25 antibody; Serine protease HTRA2 antibody; Serine protease HTRA2 mitochondrial antibody; Serine protease htra2 mitochondrial precursor antibody; Serine protease omi antibody; Serine proteinase OMI antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase S1C family.

TISSUE SPECIFICITY

Isoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.

POST-TRANSLATIONAL MODIFICATION

Autoproteolytically activated.

SUBCELLULAR LOCATION

Mitochondrion intermembrane space, Mitochondrion membrane.

FUNCTION

The human homolog of the E. Coli htrA gene product, HtrA, is identified in osteoarthritic cartilage and is repressed in SV40-transformed fibroblast. The gene encoding HtrA protein is highly conserved among mammalian species and belongs to the serine protease family. The HtrA protein contains an IGF-binding domain and exhibits endoproteolytic activity, including autocatalytic cleavage. HtrA is a secreted protein that is expressed in heterologous systems. HtrA plays a role in the degradation of denatured proteins and cell growth regulation. Human HtrA2 (also designated Omi), a novel member of the HtrA serine protease family, is highly homologous to HtrA (also known as L56 and HtrA1). HtrA2 is a ubiquitously expressed nuclear protease that is capable of autoproteolysis. The HtrA2 protein exists as two polypeptides and as an alternatively spliced form called D-Omi, which is predominately expressed in the kidney, colon and thyroid. Due to a modified PDZ domain, D-Omi does not interact with the known partner of HtrA2, the Mxi2 protein. Like HtrA, HtrA2 is involved in the degradation aberrantly folded proteins during conditions of cellular stress, suggesting that it may possess a chaperone-like role under normal conditions.