PRODUCT CODE: ET1612-83

HSPA4 Recombinant Rabbit Monoclonal Antibody [SD0861] (ET1612-83)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of HSPA4 on Hela cells lysates using anti-HSPA4 antibody at 1/1,000 dilution.
  • Western blot analysis of HSPA4 on Hela cells lysates using anti-HSPA4 antibody at 1/1,000 dilution.
  • ICC staining HSPA4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HSPA4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HSPA4 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with HSPA4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of HSPA4 on Hela cells lysates using anti-HSPA4 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

HSPA4 Recombinant Rabbit Monoclonal Antibody [SD0861] (ET1612-83)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela, HepG2, rat brain tissue, mouse brain tissue, human liver cancer tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD0861

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

94 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC/IF

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

HSPA4

SYNONYMS

APG 2 antibody; Epididymis secretory sperm binding protein Li 5a antibody; Heat shock 70 kDa protein 4 antibody; Heat shock 70 related protein APG 2 antibody; Heat shock 70-related protein APG-2 antibody; Heat shock protein, 110 kDa antibody; HEL S 5a antibody; HS24/P52 antibody; hsp70 antibody; Hsp70 RY antibody; hsp70RY antibody; HSP74_HUMAN antibody; HSPA4 antibody; HSPH2 antibody; MGC131852 antibody; RY antibody

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 70 family.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, which include the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The HSP 110 family (also known as the HSP 105 family) is composed of HSP 105, Apg-1 and Apg-2.