HSP90 Beta Mouse Monoclonal Antibody [2-1-G3] (EM21103)
Catalog# EM21103
Mouse Monoclonal to HSP90 Beta
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HepG2 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: K-562 cell lysate (20 µg/Lane)
Lane 5: Jurkat cell lysate (20 µg/Lane)
Lane 6: NIH/3T3 cell lysate (20 µg/Lane)
Lane 7: C6 cell lysate (20 µg/Lane)
Lane 8: PC-12 cell lysate (20 µg/Lane)
Lane 9: Mouse brain tissue lysate (40 µg/Lane)
Lane 10: Mouse heart tissue lysate (40 µg/Lane)
Lane 11: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 83 kDa
Observed band size: 83 kDa
Exposure time: 53 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM21103) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
HSP90 Beta Mouse Monoclonal Antibody [2-1-G3] (EM21103)
Immunogen
Synthetic peptide within human hsp90 beta aa 311-360 / 724.
Host
Mouse
Positive Control
HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, C6 cell lysate, PC-12 cell lysate, mouse brain tissue lysate, mouse heart tissue lysate, rat brain tissue lysate, human brain tissue, rat brain tissue, human colon carcinoma tissue, human tonsil tissue, mouse testis tissue, mouse brain tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
2-1-G3
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IHC-P
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1:500-1:10,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 83 kDa
Isotype
IgG2a
TARGET
UNIPROT #
PROTEIN NAME
HSP90 Beta
SYNONYMS
90 kda heat shock protein beta HSP90 beta antibody;D6S182 antibody;FLJ26984 antibody;Heat shock 84 kDa antibody;Heat shock 90kD protein 1, beta antibody;Heat shock 90kDa protein 1 beta antibody;Heat shock protein 90 alpha family class B member 1 antibody;Heat shock protein 90 kDa antibody;Heat shock protein 90kDa alpha (cytosolic) class B member 1 antibody;Heat shock protein 90kDa alpha family class B member 1 antibody;Heat shock protein beta antibody;Heat shock protein HSP 90 beta antibody;Heat shock protein HSP 90-beta antibody;HS90B_HUMAN antibody;HSP 84 antibody;HSP 90 antibody;HSP 90 b antibody;HSP 90b antibody;HSP84 antibody;HSP90 BETA antibody;hsp90ab1 antibody;HSP90B antibody;HSPC2 antibody;HSPCB antibody
SEQUENCE SIMILARITIES
Belongs to the heat shock protein 90 family.
POST-TRANSLATIONAL MODIFICATION
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).; ISGylated.; S-nitrosylated; negatively regulates the ATPase activity.; Phosphorylation at Tyr-301 by SRC is induced by lipopolysaccharide. Phosphorylation at Ser-226 and Ser-255 inhibits AHR interaction.; Methylated by SMYD2; facilitates dimerization and chaperone complex formation; promotes cancer cell proliferation.; Cleaved following oxidative stress resulting in HSP90AB1 protein radicals formation; disrupts the chaperoning function and the degradation of its client proteins.
SUBCELLULAR LOCATION
Cytoplasm
FUNCTION
Heat shock protein HSP 90-beta also called HSP90beta is a protein that in humans is encoded by the HSP90AB1 gene. HSP90AB1 is a molecular chaperone. Chaperones are proteins that bind to other proteins, thereby stabilizing them in an ATP-dependent manner. Chaperones stabilize new proteins during translation, mature proteins which are partially unstable but also proteins that have become partially denatured due to various kinds of cellular stress. In case proper folding or refolding is impossible, HSPs mediate protein degradation. They also have specialized functions, such as intracellular transport into organelles.
CITATIONS
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Min Liao
Heat Shock Protein Member 8 Is an Attachment Factor for Infectious Bronchitis Virus
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WB
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IP
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CO-IP
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chicken
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Citation
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