PRODUCT CODE: ET1612-54

Hsp75 Recombinant Rabbit Monoclonal Antibody [SD08-71] (ET1612-54)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

-
+
Western blot analysis of Hsp75 on different lysates using anti-Hsp75 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela               <br />
Lane 2: 293T
  • Western blot analysis of Hsp75 on different lysates using anti-Hsp75 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: Hela               <br />
Lane 2: 293T
Western blot analysis of Hsp75 on different lysates using anti-Hsp75 antibody at 1/1,000 dilution.
Positive control:
Lane 1: Hela
Lane 2: 293T

Applications

  • WB

  • IP

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Hsp75 Recombinant Rabbit Monoclonal Antibody [SD08-71] (ET1612-54)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

293T, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-71

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

75 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

Hsp75

SYNONYMS

Heat Shock Protein 75 antibody; Heat shock protein 75 kDa antibody; Heat shock protein 75 kDa mitochondrial antibody; HSP 75 antibody; HSP90L antibody; mitochondrial antibody; TNF receptor associated protein 1 antibody; TNFR Associated Protein antibody; TNFR-associated protein 1 antibody; TRAP-1 antibody; Trap1 antibody; TRAP1_HUMAN antibody; Tumor Necrosis Factor Receptor Associated Protein 1 antibody; Tumor necrosis factor type 1 receptor associated protein antibody; Tumor necrosis factor type 1 receptor-associated protein antibody

SEQUENCE SIMILARITIES

Belongs to the heat shock protein 90 family.

TISSUE SPECIFICITY

Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung, placenta and bladder. Expression is highly reduced in bladder cancer and renal cell carcinoma specimens compared to healthy tissues, but it is increased in other type of tumors.

SUBCELLULAR LOCATION

Mitochondrion, Mitochondrion inner membrane, Mitochondrion matrix.

FUNCTION

The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent polypeptide chains across cellular membranes and regulation of protein folding. Heat shock protein 75 mitochondrial precursor (HSP 75), also called tumor necrosis factor type 1 receptor-associated protein (TRAP1), is a 704 amino acid member of the heat shock protein 90 family. HSP 75 localizes to the mitochondrion and is expressed in a variety of tissues, including skeletal muscle, liver, heart, brain, pancreas, lung and placenta, functioning as a chaperone that expresses an ATPase activity.