Hsp70 Recombinant Rabbit Monoclonal Antibody [PD01-65] (HA721497)
Catalog# HA721497
Rabbit Monoclonal to Hsp70
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WB
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IHC-P
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IF-Tissue
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Human
Lane 1: HCT 116 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A431 cell lysate
Lane 5: A549 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721497) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Hsp70 Recombinant Rabbit Monoclonal Antibody [PD01-65] (HA721497)
Immunogen
Recombinant fragment within human hsp70 aa 50-300.
Host
Rabbit
Positive Control
HCT 116 cell lysate, HEK-293 cell lysate, HepG2 cell lysate, A431 cell lysate, A549 cell lysate, human breast carcinoma tissue, human liver carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PD01-65
RRID
APPLICATION DILUTION
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WB
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1:1,000
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IHC-P
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1:200
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IF-Tissue
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 70 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Hsp70
SYNONYMS
DnaK type molecular chaperone HSP70 1 antibody;Epididymis secretory protein Li 103 antibody;FLJ54303 antibody;FLJ54370 antibody;FLJ54392 antibody;FLJ54408 antibody;FLJ75127 antibody;Heat shock 70 kDa protein 1 antibody;Heat shock 70 kDa protein 1/2 antibody;Heat shock 70 kDa protein 1A/1B antibody;Heat shock 70kDa protein 1A antibody;Heat shock 70kDa protein 1B antibody;Heat shock induced protein antibody;HEL S 103 antibody;HSP70 1 antibody;HSP70 1B antibody;HSP70 2 antibody;HSP70-1/HSP70-2 antibody;HSP70-1A antibody;HSP70.1 antibody;HSP70.1/HSP70.2 antibody;HSP70I antibody;HSP71_HUMAN antibody;HSP72 antibody;HSPA1 antibody;HSPA1A antibody;HSPA1B antibody
SEQUENCE SIMILARITIES
Belongs to the heat shock protein 70 family.
TISSUE SPECIFICITY
HSPA1B is testis-specific.
POST-TRANSLATIONAL MODIFICATION
In response to cellular stress, acetylated at Lys-77 by NA110 and then gradually deacetylated by HDAC4 at later stages. Acetylation enhances its chaperone activity and also determines whether it will function as a chaperone for protein refolding or degradation by controlling its binding to co-chaperones HOPX and STUB1. The acetylated form and the non-acetylated form bind to HOPX and STUB1 respectively. Acetylation also protects cells against various types of cellular stress.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
The 70 kilodalton heat shock proteins (Hsp70s) are a family of conserved ubiquitously expressedheat shock proteins. Proteins with similar structure exist in virtually all living organisms. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. When not interacting with a substrate peptide, Hsp70 is usually in an ATP bound state. Hsp70 by itself is characterized by a very weak ATPase activity, such that spontaneous hydrolysis will not occur for many minutes. As newly synthesized proteins emerge from the ribosomes, the substrate binding domain of Hsp70 recognizes sequences of hydrophobic amino acid residues, and interacts with them. This spontaneous interaction is reversible, and in the ATP bound state Hsp70 may relatively freely bind and release peptides. However, the presence of a peptide in the binding domain stimulates the ATPase activity of Hsp70, increasing its normally slow rate of ATP hydrolysis.
CITATIONS
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Interplay between ferric uptake regulator Fur and horizontally acquired virulence regulator EsrB coordinates virulence gene expression in Edwardsiella piscicida
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WB
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Human
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Citation
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