PRODUCT CODE: EM00704

HSP60 Mouse Monoclonal Antibody [1-80] (EM00704)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of HSP60 on different cell lysates using anti-HSP60 antibody at 1/2000 dilution.<br />
Positive control:   <br />
Lane 1: Hela               <br />
Lane 2: HepG2 <br />
Lane 3: Jurkat             <br />
Lane 4: PC12 <br />
Lane 5: F9
  • Western blot analysis of HSP60 on different cell lysates using anti-HSP60 antibody at 1/2000 dilution.<br />
Positive control:   <br />
Lane 1: Hela               <br />
Lane 2: HepG2 <br />
Lane 3: Jurkat             <br />
Lane 4: PC12 <br />
Lane 5: F9
  • ICC staining HSP60 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-HSP60 antibody. Counter stained with hematoxylin.
Western blot analysis of HSP60 on different cell lysates using anti-HSP60 antibody at 1/2000 dilution.
Positive control:
Lane 1: Hela
Lane 2: HepG2
Lane 3: Jurkat
Lane 4: PC12
Lane 5: F9

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

HSP60 Mouse Monoclonal Antibody [1-80] (EM00704)

Immunogen

Peptide

Host

Mouse

Positive Control

Hela, HepG2, Jurkat, PC12, F9, human breast cancer tissue, human kidney tissue, mouse kidney tissue, mouse heart tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

1-80

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

61kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:2,000

  • ICC

  • 1:200

  • IHC-P

  • 1:200

TARGET

UNIPROT #

PROTEIN NAME

HSP60

SYNONYMS

60 kDa chaperonin antibody; 60 kDa heat shock protein, mitochondrial antibody; CH60_HUMAN antibody; Chaperonin 60 antibody; Chaperonin, 60-KD antibody; CPN60 antibody; fa04a05 antibody; GROEL antibody; heat shock 60kDa protein 1 (chaperonin) antibody; Heat shock protein 1 (chaperonin) antibody; Heat shock protein 60 antibody; Heat shock protein 65 antibody; heat shock protein family D (Hsp60) member 1 antibody; HLD4 antibody; Hsp 60 antibody; HSP 65 antibody; HSP-60 antibody; HSP60 antibody; HSP65 antibody; HSPD1 antibody; HuCHA60 antibody; Mitochondrial matrix protein P1 antibody; P60 lymphocyte protein antibody; short heat shock protein 60 Hsp60s1 antibody; SPG14 antibody

SEQUENCE SIMILARITIES

Belongs to the chaperonin (HSP60) family.

SUBCELLULAR LOCATION

Mitochondrion matrix

FUNCTION

The 60 kDa heat shock protein (HSP60) is a highly conserved protein that acts as a chaperone involved in correct folding of newly synthesized proteins. Mainly expressed in mitochondria, HPS60 has also been found in the cytosol of normal heart and muscle cells. The HSP60 proteins are abundant in the cell and are highly conserved between bacteria and human, with 50%-61% sequence homology.