HSP60 Mouse Monoclonal Antibody [1-80] (EM00704)
Catalog# EM00704
Mouse Monoclonal to HSP60
Tested for
-
WB
-
ICC
-
IHC-P
React with
-
Human
-
Mouse
-
Rat
Western blot analysis of HSP60 on different lysates with Mouse anti-HSP60 antibody (EM00704) at 1/500 dilution.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: PC-12 cell lysate
Lane 5: F9 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM00704) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: PC-12 cell lysate
Lane 5: F9 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 30 seconds;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM00704) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
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