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PRODUCT CODE: R1511-11

HSP47 Rabbit Polyclonal Antibody (R1511-11)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of HSP47 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1511-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Rat placenta tissue lysate<br /> Lane 2: Siha cell lysate<br /> Lane 3: NIH/3T3 cell lysate
  • Western blot analysis of HSP47 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1511-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: Rat placenta tissue lysate<br /> Lane 2: Siha cell lysate<br /> Lane 3: NIH/3T3 cell lysate
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HSP47 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1511-11, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of HSP47 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1511-11, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Rat placenta tissue lysate
Lane 2: Siha cell lysate
Lane 3: NIH/3T3 cell lysate

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

HSP47 Rabbit Polyclonal Antibody (R1511-11)

Immunogen

Recombinant protein within mouse hsp47 aa 50-300.

Host

Rabbit

Positive Control

Rat placenta tissue lysate, Siha cell lysate, NIH/3T3 cell lysate, human placenta tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

46 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P50454

PROTEIN NAME

HSP47

SYNONYMS

47 kDa heat shock protein antibody; 47 kDa heat shock protein precursor antibody; Arsenic transactivated protein 3 antibody; Arsenic-transactivated protein 3 antibody; AsTP 3 antibody; AsTP3 antibody; CBP 1 antibody; CBP 2 antibody; CBP1 antibody; CBP2 antibody; Cell proliferation-inducing gene 14 protein antibody; Collagen binding protein 1 antibody; Collagen binding protein 2 antibody; Collagen binding protein antibody; Collagen-binding protein 2 antibody; Collagen-binding protein antibody; Colligen antibody; Colligin 1 antibody; Colligin 2 antibody; Colligin antibody; colligin-1 antibody; colligin-2 antibody; gp46 antibody; Heat shock protein 47 antibody; Heat-shock protein 47 antibody; HGNC 1547 antibody; Hsp 47 antibody; J6 antibody; OI10 antibody; PIG 14 antibody; PIG14 antibody; PPROM antibody; Proliferation inducing gene 14 antibody; Proliferation inducing gene 14 protein antibody; RA A47 antibody; RA-A47 antibody; Rheumatoid arthritis antigen A 47 antibody; rheumatoid arthritis antigen A-47 antibody; Rheumatoid arthritis related antigen RA A47 antibody; Rheumatoid arthritis-related antigen RA-A47 antibody; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody; serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 2, (collagen-binding protein 2) antibody; Serine or cysteine proteinase inhibitor clade H member 1 antibody; Serine or cysteine proteinase inhibitor clade H member 2 antibody; SERPH_HUMAN antibody; Serpin H1 antibody; Serpin peptidase inhibitor clade H member 1 antibody; serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) antibody; Serpin peptidase inhibitor, clade H, member 1 antibody; SERPINH1 antibody; SERPINH2 antibody

SEQUENCE SIMILARITIES

Belongs to the serpin family.

SUBCELLULAR LOCATION

Endoplasmic reticulum.

FUNCTION

Heat shock protein 47, also known as SERPINH1 is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. HSP47 is expressed by cells present in Endoplasmic Reticulum. These cells synthesize and secrete type I and type II collagen. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis. Heat shock protein 47 has been shown to interact with collagens I, II, III, IV and V. In the ER, HSP47 interacts with and stabilizes correctly-folded procollagen. Nucleotide polymorphisms may be associated with preterm birth and Osteogenesis Imperfecta type X. Serpin-H1 is up-regulated in several cancers including squamous cell carcinoma, breast and prostate carcinomas. Expression in tumors drives malignant growth and invasion by enhancing deposition of extracellular matrix proteins.