HSD3B1 Recombinant Rabbit Monoclonal Antibody [PSH18-08] (HA723971)
Catalog# HA723971
Rabbit Monoclonal to HSD3B1
Tested for
-
WB
-
IHC-P
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HSD3B1 on different lysates with Rabbit anti-HSD3B1 antibody (HA723971) at 1/5,000 dilution.
Lane 1: Caco-2 cell lysate (20 µg/Lane)
Lane 2: Caco-2 cell lysate (no heat) (20 µg/Lane)
Lane 3: Mouse ovary tissue lysate (20 µg/Lane)
Lane 4: Rat ovary tissue lysate (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723971) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: Caco-2 cell lysate (20 µg/Lane)
Lane 2: Caco-2 cell lysate (no heat) (20 µg/Lane)
Lane 3: Mouse ovary tissue lysate (20 µg/Lane)
Lane 4: Rat ovary tissue lysate (20 µg/Lane)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: Lane 1-2: 20 seconds; Lane 3-4: 1 minute 16 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723971) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.