HPRT Recombinant Rabbit Monoclonal Antibody [JU03-26] (ET1706-08)
Catalog# ET1706-08
Rabbit Monoclonal to HPRT
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
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Zebrafish
Lane 1: MCF-7 cell lysate
Lane 2: 293 cell lysate
Lane 3: Hela cell lysate
Lane 4: A431 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-08) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
HPRT Recombinant Rabbit Monoclonal Antibody [JU03-26] (ET1706-08)
Immunogen
Synthetic peptide within human hprt aa 169-218 / 218.
Host
Rabbit
Positive Control
MCF-7 cell lysate, 293 cell lysate, Hela cell lysate, A431 cell lysate, rat kidney tissue lysate, rat tonsil brain lysate, mouse testis tissue lysate, mouse colon tissue lysate, zebrafish tissue lysates, rat brain tissue, human tonsil tissue, human colon carcinoma tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU03-26
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
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IHC-P
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1:50-1:200
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
25 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
HPRT
SYNONYMS
HGPRT antibody;HGPRTase antibody;HPRT 1 antibody;HPRT_HUMAN antibody;HPRT1 antibody;Hypoxanthine guanine phosphoribosyltransferase antibody;Hypoxanthine phosphoribosyltransferase 1 (Lesch Nyhan syndrome) antibody;Hypoxanthine phosphoribosyltransferase 1 antibody;Hypoxanthine-guanine phosphoribosyltransferase antibody
SEQUENCE SIMILARITIES
Belongs to the purine/pyrimidine phosphoribosyltransferase family.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
HPRT (hypoxanthine phosphoribosyltransferase 1), also known as HGPRT or HPRT1, is a 218 amino acid cytoplasmic protein that belongs to the purine/pyrimidine phosphoribosyltransferase family. Involved in purine metabolism, HPRT functions as a purine salvage enzyme that catalyzes the conversion of hypoxathine and guanine to their respective mononucleotides (inosine monophosphate and guanosine monophosphate, respectively). HPRT exists as a homotetramer that can bind two magnesium ions as cofactors. Defects in the gene encoding HPRT are the cause of gout and Lesch-Nyhan syndrome (LNS), both of which are characterized by a partial or complete lack of NPRT enzymatic activity. While a partial loss of HPRT enzymatic activity results in a buildup of uric acid (gout), a total loss of enzymatic activity results in hyperuricaemia, mental retardation, choreoathetosis and compulsive self-mutilation, all of which are symptoms associated with LNS. The severity of these diseases suggests an essential role for HPRT in purine metabolism.
CITATIONS
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Nanxin Zhang,et al
Effects and mechanisms of Polygonati Rhizoma polysaccharide on potassium oxonate and hypoxanthine-induced hyperuricemia in mice
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WB
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Mouse
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Citation
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