PRODUCT CODE: ET1706-02

HP1 gamma Recombinant Rabbit Monoclonal Antibody [JU81-36] (ET1706-02)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of HP1 gamma on PC-12 (1) and MCF-7 (2) cell lysate using anti-HP1 gamma antibody at 1/500 dilution.
  • Western blot analysis of HP1 gamma on PC-12 (1) and MCF-7 (2) cell lysate using anti-HP1 gamma antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-HP1 gamma antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with HP1 gamma antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of HP1 gamma on PC-12 (1) and MCF-7 (2) cell lysate using anti-HP1 gamma antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

HP1 gamma Recombinant Rabbit Monoclonal Antibody [JU81-36] (ET1706-02)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, MCF-7, rat testis tissue, human tonsil tissue, human placenta tissue, mouse colon tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JU81-36

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

20 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

HP1 gamma

SYNONYMS

CBX 3 antibody; CBX3 antibody; CBX3_HUMAN antibody; Chromobox homolog 3 (HP1 gamma homolog, Drosophila) antibody; Chromobox homolog 3 antibody; Chromobox protein homolog 3 antibody; GAMMA antibody; HECH antibody; Heterochromatin like protein 1 antibody; Heterochromatin protein 1 homolog gamma antibody; Heterochromatin protein HP1 gamma antibody; HP1 gamma antibody; HP1 gamma homolog antibody; HP1Hs gamma antibody; Modifier 2 protein antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylated by PIM1. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication. The p150 subunit of CAF-1 also supports the maintenance of heterochromatin, which requires the synthesis of both new histones and heterochromatin proteins and their orderly assembly during DNA replication. Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of Histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated.