PRODUCT CODE: M1211-2

HP1 alpha Mouse Monoclonal Antibody [B0-1] (M1211-2)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis on MCF-7 cell lysates using anti-HP-1αMouse mAb (Cat. # M1211-2).
  • Western blot analysis on MCF-7 cell lysates using anti-HP-1αMouse mAb (Cat. # M1211-2).
  • Immunofluorescent staining of MCF-7 cells using anti-HP-1α Mouse mAb (Cat. # M1211-2).
Western blot analysis on MCF-7 cell lysates using anti-HP-1αMouse mAb (Cat. # M1211-2).

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

HP1 alpha Mouse Monoclonal Antibody [B0-1] (M1211-2)

Immunogen

Recombinant protein

Host

Mouse

Positive Control

MCF-7

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

B0-1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

22kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:5,000

  • ICC

  • 1:500

TARGET

UNIPROT #

PROTEIN NAME

HP1 alpha

SYNONYMS

Antigen p25 antibody; CBX5 antibody; CBX5_HUMAN antibody; CG8409 antibody; Chromobox 5 antibody; Chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody; Chromobox homolog 5 antibody; Chromobox protein homolog 5 antibody; Epididymis luminal protein 25 antibody; HEL25 antibody; Heterochromatin protein 1 alpha antibody; Heterochromatin protein 1 antibody; Heterochromatin protein 1 homolog alpha antibody; HP1 alpha antibody; HP1 alpha homolog antibody; HP1 antibody; HP1A antibody; HP1Hs alpha antibody; Su(var)205 antibody

POST-TRANSLATIONAL MODIFICATION

Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle (By similarity). Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis.; Ubiquitinated.

SUBCELLULAR LOCATION

Nucleus

FUNCTION

The family of Heterochromatin Protein 1 (HP1) ("Chromobox Homolog", CBX) are highly conserved adapter proteins, which have important functions in the cell nucleus. These functions include gene repression by heterochromatin formation, transcriptional activation, regulation of binding of cohesion complexes to centromere, sequesteration of genes to nuclear periphery, transcriptional arrest, maintenance of heterochromatin integrity, gene repression at single nucleosome level and gene repression by heterochromatization of euchromatin.Three different paralogs of HP1 are found in mammals, HP1α, HP1βand HP1γ.